Generator

Part:BBa_K1833004

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-16)


lac promoter -> RpoA-N-terminus fused to Gal4

This part encodes the fusion protein consisting of the RpoA-N-terminus and Gal4. The part has a lacUV5 promoter, so the production of protein can be induced with IPTG in any E. coli strain. RpoA is the gene encoding the alpha subunit of E. coli RNA Polymerase, and the N-terminal domain is responsible for assembly of RNAP, while the C-terminal domain is responsible for recruiting the polymerase to DNA. This fusion protein is used as part of a bacterial two-hybrid system, where the Gal4 binds to Gal11P as in the yeast 2-hybrid system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 276
    Illegal XhoI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 105


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None